کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4065 206 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new salt inducible expression system for Lactococcus lactis
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A new salt inducible expression system for Lactococcus lactis
چکیده انگلیسی

A new expression system for Lactococcus lactis based on the salt inducible BusA promoter and the BusR repressor gene of L. lactis MG1363 was developed. To achieve salt induction, the expression of BusR was modulated by introducing mutations to its promoter sequence. An activity of 6.0 μkat l−1 of the model enzyme Lactobacillus amylovorus α-amylase was achieved in the bioreactor cultivation. The major advantage of the current expression system is that no additions of inducing agents are needed into bioreactor cultivations.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 48, Issue 1, 15 December 2009, Pages 132–135
نویسندگان
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