Isolation and Induction of Differentiation of Seine Adipose-Derived Mesenchymal Stem Cells

ObjectivesTo establish a method for high yield mesenchymal stem cells collection, as well as a culture method for identifying mesenchymal stem cells from the swine adipose-derived mesenchymal stem cell (ADMSC).MethodsSwine ADMSCs were isolated from fat tissue with collagenase, followed by induction of differentiation to osteogenic, adipogenic and chondrogrnic cells. The survival curve of the ADMSC at the 37°C and 38°C were measured using WST-1Cell Proliferation Assay Reagent.ResultADMSCs isolated with collagenase from swine neck fat tissue generated a stable uniform appearance after the second generation. The passage period was five days. ADMSC could differentiate into osteogenic, adipogenic or chondrogrnic cells under different culture conditions. The highest growth rate was achieved at 38°C in this study.ConclusionSwine ADMSCs have the potential to differentiate into osteogenic, adipogenic or chondrogrnic cells, and they may be appropriate for transplantation for both research and clinical purpose.