کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4328860 | 1614194 | 2008 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of a nervous system-specific promoter for growth-associated protein 43 gene in Medaka (Oryzias latipes)
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کلمات کلیدی
DRGMGDDPTGAP-43PKCBPShpfMCseGFPDPFTBPC/EBP - C / EBPWISH - آرزو کردنTranscriptional regulation - تنظیم ترانزیتیbase pairs - جفت پایهCNS - دستگاه عصبی مرکزیMultiple cloning sites - سایت های کلونینگ چندگانهcentral nervous system - سیستم عصبی مرکزیperipheral nervous system - سیستم عصبی پیرامونیEvolution - فرگشت CCAAT-enhancer binding protein - پروتئین اتصال دهنده تقویت کننده CCAATenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استTATA-binding protein - پروتئین متصل به TATAgrowth-associated protein 43 - پروتئین مرتبط با رشد 43Protein kinase C - پروتئین کیناز سیPromoter - پروموترPNS - کارمندان دولتwhole-mount in situ hybridization - کلیدی در هیبریداسیون in situ
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
Genes expressed by neurons are controlled by specific, interacting cis-regulatory elements and trans-acting factors within their promoters. In the present study, we asked whether the transcriptional machinery regulating neuron-specific gene expression was conserved in evolution. We identified a GAP-43 homolog in Medaka (Oryzias latipes), and analyzed its expression during various stages of development. Compared with the amino acid sequences of GAP-43 homologs in other vertebrates, the amino-terminus of GAP-43 was highly conserved evolutionarily, but the carboxy-terminus exhibited significant variability. Expression of GAP-43 predominantly occurred in cells of the central and peripheral nervous systems as determined by in situ hybridization and by RT-PCR. Expression of GAP-43 increased throughout development and significant levels continued to be expressed into adulthood. We also showed that a proximal â¼Â 2.0 kbp fragment in the 5â²-flanking region had promoter activity as determined by in vivo reporter assays. Furthermore, based upon computational analysis of transcription factor binding sites and an in vivo reporter analysis using sequentially deleted promoters, we demonstrated that cis-regulatory elements for neuronal expression were widely distributed in this region. In mammals, a TATA-box, E-box and neuronal repressive elements have been thought to contribute to neuronal expression. However, these features were not found in the orthologous region of the Medaka GAP-43 promoter. Our results suggest that the arrangement of cis-regulatory elements of the GAP-43 ortholog in Medaka is different from that in mammals, yet maintains neuron-specific regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1245, 15 December 2008, Pages 1-15
Journal: Brain Research - Volume 1245, 15 December 2008, Pages 1-15
نویسندگان
Kazuhiro E. Fujimori, Takashi Kawasaki, Tomonori Deguchi, Syunsuke Yuba,