کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4329769 | 1614231 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
TAT-PAX6 protein transduction in neural progenitor cells: A novel approach for generation of dopaminergic neurones in vitro
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کلمات کلیدی
6-OHDAgrowth factor(s)GDF5transactivator of transcriptionFGF8CRLHSV-1SVZPax6GDNFPTDGFPGFAP6-HydroxydopamineEGFbFGFNSCventral mesencephalon - mesencephalon شکمیMPP+ - MPP +Parkinson's disease - بیماری پارکینسونTAT - تاتDifferentiation - تفکیکsubstantia nigra - توده سیاهtyrosine hydroxylase - تیروزین هیدروکسیلازShh - خیرProtein transduction domain - دامنه انتقال پروتئینDopaminergic - دوپامینرژیکembryonic day - روز جنینیNeural stem cell - سلول های بنیادی عصبیneural progenitor cell - سلول پیشرو عصبیsonic hedgehog - صدای جیر جیرcrown-rump length - طول تاج و تختGrowth/differentiation factor 5 - عامل رشد / تمایز 5Fibroblast growth factor 8 - عامل رشد فیبروبلاست 8basic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهAntp - مزهsubventricular zone - منطقه فرعیNeurosphere - نوروسفرhemagglutinin - هماگلوتینینHIV-1 - ویروس اچ آی وی نوع یکhuman immunodeficiency virus 1 - ویروس نقص ایمنی بدن 1Herpes simplex virus 1 - ویروس هرپس سیمپلکس 1Glial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالgreen fluorescent protein - پروتئین فلورسنت سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Neural stem cells (NSCs) have the potential to be used for the treatment of Parkinson's disease (PD), as they can be expanded, manipulated and differentiated in vitro to generate dopaminergic neurones which are suitable for transplantation. Since NSCs have a tendency to follow an astrocytic lineage after differentiation in vitro, researchers are investigating ways to induce a neuronal phenotype in these cells. In this study, the human immunodeficiency virus 1 (HIV-1) transactivator of transcription (TAT) protein transduction domain (PTD) system was used in an attempt to promote neuronal differentiation in rodent NSCs. A fusion protein that incorporated both the TAT PTD and the Pax6 protein (a determinant of neurogenesis) was created and added to the differentiation phase of embryonic day (E) 12 rat ventral mesencephalic (VM) neurosphere cultures. Subsequently, application of dopaminergic growth factors (GFs) was used in an attempt to induce the newly-generated neuronal progenitors to adopt a dopaminergic phenotype. In addition, a technique involving the differentiation of intact neurospheres (instead of the differentiation of neurosphere-derived dissociated cells) was investigated for its ability to promote dopaminergic neurogenesis. Immunocytochemical analysis of the differentiated neurosphere cultures indicated that both of these techniques had a significant effect on the emergence of dopaminergic neurones. Moreover, upon combination of these techniques, a further increase in dopaminergic neuronal generation was observed. Based on the findings of the present study, it is clear that NSCs are greatly influenced by their environment and that optimised in vitro conditions can support the potential of these cells to differentiate into dopaminergic neurones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1208, 7 May 2008, Pages 25-34
Journal: Brain Research - Volume 1208, 7 May 2008, Pages 25-34
نویسندگان
Katie Spitere, André Toulouse, David B. O'Sullivan, Aideen M. Sullivan,