کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4331589 | 1614305 | 2007 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative proteomic profiling of membrane proteins from the mouse brain cortex, hippocampus, and cerebellum using the HysTag reagent: Mapping of neurotransmitter receptors and ion channels
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کلمات کلیدی
FTICRLTQTOF2DE - 2deMS/MS - MS / MStwo dimensional electrophoresis - الکتروفورز دو بعدیFourier transform - تبدیل فوریهQuantitative analysis - تجزیه و تحلیل کمیFourier transform ion cyclotron resonance - رزونانس سیکلوترون یون تبدیل تبدیل فوریهtime of flight - زمان پروازMass spectrometry - طیف سنجی جرمیTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیPlasma membrane - غشای پلاسماMALDI TOF - مالدی TOFMembrane protein - پروتئین غشائیliquid chromatography - کروماتوگرافی مایعneurotransmitter receptor - گیرنده نوروترانسمیترIon-channel - یون کانال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Analysis of the brain proteome and studying brain diseases through clinical biopsies and animal disease models require methods of quantitative proteomics that are sensitive and allow identification and quantification of low abundant membrane proteins from minute amount of tissue. Taking advantage of recently developed methods for isolation of membrane proteins from 10-20Â mg brain tissue [Nielsen, P.Aa., Olsen, J.V., Podtelejnokov, A.V., Andersen, J.R., Mann, M., WiÅniewski, J.R., 2005. Proteomic mapping of brain plasma membrane proteins. Mol. Cell. Proteomics 4, 402--408] and the HysTag-quantification method [Olsen, J.V., Andersen, J.R., Nielsen, P.Aa., Nielsen, M.L., Figeys, D., Mann, M., WiÅniewski, J.R., 2004. HysTag---A novel proteomic qualification tool applied to differential analysis of membrane proteins from distinct areas of mouse brain. Mol. Cell. Proteomics 3, 82--92] we performed quantitative proteomic analysis of three functionally distinct compartments of mouse brain: cortex, hippocampus, and cerebellum. In total, 976 unique peptides corresponding to 555 unique proteins were quantified. Up to 20-fold differences in the levels of some proteins between brain areas were measured. For many quantified proteins - as for glutamate receptors, calcium channel subunits, and ATP-ases - an excellent correlation between our proteomic data and previously published mRNA expression levels or intensity of immunostaining was found. Our results clearly demonstrate differences in levels of membrane proteins mapped in distinct brain compartments and offer a technology that allows in depth study of brain membrane proteomes, such as mouse models of neurological diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1134, 23 February 2007, Pages 95-106
Journal: Brain Research - Volume 1134, 23 February 2007, Pages 95-106
نویسندگان
Jesper V. Olsen, Peter Aa. Nielsen, Jens R. Andersen, Matthias Mann, Jacek R. WiÅniewski,