کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4337936 | 1614832 | 2013 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Periadolescent ethanol vapor exposure persistently reduces measures of hippocampal neurogenesis that are associated with behavioral outcomes in adulthood
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کلمات کلیدی
DcxBromo-deoxy-Uridineblood ethanol concentrationsBECsNIHNPCSVZFluoro-Jade BGCLFJBDTIFSTPDSPBSforced swim test - آزمون شناور اجباریMRI - امآرآی یا تصویرسازی تشدید مغناطیسیImmunoreactivity - ایمنی فعالImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیBrdU - بروموداکسی اوریدینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of variancediffusion tensor imaging - تصویربرداری تانسور انتشارMagnetic resonance imaging - تصویربرداری رزونانس مغناطیسیdoublecortin - دوچرخهpostnatal days - روزهای پس از تولدNeural progenitor cells - سلولهای پیش گیاه عصبیalcohol exposure - قرار گرفتن در معرض الکلgranule cell layer - لایه سلول گرانولNIH, National Institutes of Health - مؤسسه ملی سلامتPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریAdolescent - نوجوانیNeurogenesis - نوروژنزHippocampal dentate gyrus - هیپوکامپ دندانه دارStartle - وحشت زده شدن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Excessive alcohol consumption is prevalent among adolescents and may result in lasting neurobehavioral consequences. The use of animal models to study adolescent alcohol exposure has the advantage of allowing for the control necessary in order to evaluate the effects of ethanol on the brain and separate such effects from genetic background and other environmental insults. In the present study the effects of moderate ethanol vapor exposure, during adolescence, on measures of neurogenesis and behavioral measures were evaluated at two different times following ethanol withdrawal, in adulthood. The two groups of Wistar rats were both exposed to intermittent ethanol vapor (14Â h on/10Â h off/day) for 35-36Â days from PD 23 to PD 58 (average blood ethanol concentration: 163Â mg%). In the first group, after rats were withdrawn from vapor they were subsequently assessed for locomotor activity, conflict behavior in the open field, and behaviors in the forced swim test (FST) and then sacrificed at 72Â days of age. The second group of rats were withdrawn from vapor and injected for 5Â days with Bromo-deoxy-Uridine (BrdU). Over the next 8Â weeks they were also assessed for locomotor activity, conflict behavior in the open field, and behaviors in the FST and then sacrificed at 113/114Â days of age. All rats were perfused for histochemical analyses. Ethanol vapor-exposed rats displayed hypoactivity in tests of locomotion and less anxiety-like and/or more “disinhibitory” behavior in the open field conflict. Quantitative analyses of immunoreactivity revealed a significant reduction in measures of neurogenesis, progenitor proliferation, as indexed by doublecortin (DCX), Ki67, and increased markers of cell death as indexed by cleaved caspase-3, and Fluoro-Jade at 72Â days, and decreases in DCX, and increases in cleaved caspase-3 at 114Â days in the ethanol vapor-exposed rats. Progenitor survival, as assessed by BrdU+, was reduced in the vapor-exposed animals that were sacrificed at 114Â days. The reduction seen in DCX labeled in cell counts was significantly correlated with hypoactivity at 24Â h after withdrawal as well as less anxiety-like and/or more “disinhibitory” behavior in the open field conflict test at 2 and 8Â weeks following termination of vapor exposure. These studies demonstrate that behavioral measures of disinhibitory behavior correlated with decreases in neurogenesis are all significantly and persistently impacted by periadolescent ethanol exposure and withdrawal in Wistar rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 244, 6 August 2013, Pages 1-15
Journal: Neuroscience - Volume 244, 6 August 2013, Pages 1-15
نویسندگان
C.L. Ehlers, W. Liu, D.N. Wills, F.T. Crews,