کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341202 | 1295827 | 2007 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Axonal lesion-induced microglial proliferation and microglial cluster formation in the mouse
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کلمات کلیدی
o.n.macrophage antigen-1FBSdays post-lesionFITCDPLHPLTBSICAM-1CD34LFA-1DAPIIBA14′,6′-diamidino-2-phenylindole - 4 '، 6'-diamidino-2-phenylindoleLymphocyte function-associated antigen-1 - آنتی ژن 1 مرتبط با عملکرد لنفوسیتStreptavidin - استرپتاویدینaxotomy - اگزوتیومBrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceTris-buffered saline - تریس بافر شورcluster of differentiation - خوشه تمایزfetal bovine serum - سرم جنین گاوalkaline-phosphatase - فسفاتاز قلیاییfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتFluoro-Jade - فلورا جیدPerforant pathway - مسیر پرفورنتionized calcium binding adaptor molecule 1 - مولکول آداپتور اتصال دهنده کلسیم یونیزه 1intercellular adhesion molecule-1 - مولکول چسبندگی بین سلولی -1Mac-1 - مک 1Hippocampus - هیپوکامپ Antibody - پادتَن یا آنتیبادیovernight - یک شب
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Microglia are innate immune cells and form the first line of defense of the CNS. Proliferation is a key event in the activation of microglia in acute pathology, and has been extensively characterized in rats, but not in mice. In this study we investigated axonal-lesion-induced microglial proliferation and surface antigen expression in C57BL/6 mice. Transection of the entorhino-dentate perforant path projection results in an anterograde axonal and a dense terminal degeneration that induces a region-specific activation of microglia in the dentate gyrus. Time-course analysis showed activation of microglial cells within the first week post-lesion and cell counting demonstrated a significant 1.6-fold increase in microglial numbers 24 h post-lesion reaching a maximal 3.8-fold increase 3 days post-lesion compared with controls. Double staining for the microglial macrophage antigen-1 and the proliferation marker bromodeoxyuridine, injected 1 h prior to perfusion, showed that lesion-reactive microglia accounted for the vast majority of proliferating cells. Microglia proliferated as soon as 24 h after lesion and 25% of all microglial cells were proliferating 3 days post-lesion. Immunofluorescence double staining showed that most activated, proliferating microglia occurred in multicellular clusters and co-expressed the intercellular adhesion molecule-1 and the hematopoietic stem cell marker cluster of differentiation 34. In conclusion, this study extends observations of axonal lesion-induced microglial proliferation in rats to mice, and provides new information on early microglial proliferation and microglial cluster formation and surface antigen expression in the mouse.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 149, Issue 1, 12 October 2007, Pages 112-122
Journal: Neuroscience - Volume 149, Issue 1, 12 October 2007, Pages 112-122
نویسندگان
L. Dissing-Olesen, R. Ladeby, H.H. Nielsen, H. Toft-Hansen, I. Dalmau, B. Finsen,