کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4341674 | 1295843 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EGFdibutyryl cAMPdbcAMPSCNMAP2RHTAVPCNPaseNBMN-methyl-d-aspartateNMDAbFGFGFAPFBSDEXMAPK - MAPKDexamethasone - دگزامتازونCircadian rhythm - ریتم شبانه روزیfetal bovine serum - سرم جنین گاوepidermal growth factor - عامل رشد اپیدرمیbasic fibroblast growth factor - فاکتور رشد فیبروبلاست پایهneurobasal medium - محیط عصبیSuprachiasmatic nucleus - هستههای سوپراکیاسماتیکNR2B subunit - واحد NR2Barginine vasopressin - وازوپرسین آرژینینGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالmicrotubule-associated protein 2 - پروتئین مرتبط با میکروتوبول 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus A novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus](/preview/png/4341674.png)
چکیده انگلیسی
The suprachiasmatic nucleus of the anterior hypothalamus is the center of an internal biological clock in mammals. Glutamate is the neurotransmitter of retino-hypothalamic tract responsible for mediating the circadian actions of light in rodents. N-methyl-d-aspartate receptors, particularly NR2B subunit are reported to be principally involved in photic resetting of the biological clock in vivo and in slice culture. But, the precise cellular mechanisms of the resetting are not elucidated, because no adequate neuronal cell lines derived from the suprachiasmatic nucleus have been established. We established a neuronal cell line, N14.5, derived from the suprachiasmatic nucleus of a transgenic rat harboring the temperature-sensitive simian virus 40 large T-antigen gene. When the cells were cultured at 39 °C, the morphological features were turned fibroblastic into neuronal round cell body with neurite extensions. These cells showed immunoreactivities for neuronal markers (βIII-tubulin, microtubule-associated protein 2 and TAU2) and as well as for vasoactive intestinal peptide which is expressed in the ventrolateral region of the suprachiasmatic nucleus. The cells expressed N-methyl-d-aspartate receptors, particularly NR1 and NR2B subunits as revealed by quantitative PCR. N-methyl-d-aspartate activated phosphorylation of p44/42 mitogen-activated protein kinase and increased expression level of Per1 and Per2 mRNA. These results suggest that the N14.5 is a novel neuronal cell line derived from the ventrolateral region of the suprachiasmatic nucleus, and that N-methyl-d-aspartate receptors expressed in the cells are a functional receptor. The N14.5 cells may be a useful tool to elucidate numerous chronobiological processes, especially resetting mechanism induced by an external light signal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 140, Issue 3, 2006, Pages 849-856
Journal: Neuroscience - Volume 140, Issue 3, 2006, Pages 849-856
نویسندگان
T. Matsushita, Y. Amagai, K. Terai, T. Kojima, M. Obinata, S. Hashimoto,