Activation of peripheral P2X receptors is sufficient to induce central sensitization in rat medullary dorsal horn nociceptive neurons

► ATP contributes to the development of central sensitization (CS) in medullary nociceptive neurons. ► Application of purinergic agonist to the rat tooth pulp induces CS in medullary nociceptive neurons. ► Central sensitization can be blocked by pulp application of the purinergic antagonist TNP-ATP. ► Central sensitization can be attenuated by medullary application of TNP-ATP.