کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4344653 1296671 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mechanical injured neurons stimulate astrocytes to express apolipoprotein E through ERK pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Mechanical injured neurons stimulate astrocytes to express apolipoprotein E through ERK pathway
چکیده انگلیسی

To explore the possible cellular source and mechanism of apolipoprotein E (apoE) expression in mechanical injured neuronal cultures. Primary cultured mouse cortical neurons were subjected into mechanical injury by needle scratching. The conditioned medium of wild type (WT) primary mouse astrocytes was collected and added into cultured injured apoE knockout (KO) neurons. Separately, the conditioned medium of injured apoE KO neurons was collected and added into cultured WT astrocytes. We used a specific inhibitor of extracellular signal-regulated kinase (ERK) to block the possible apoE-associated pathway between injured neurons and astrocytes. The apoE expression levels of the cells and secreted into medium were measured by Western blot, respectively. The apoE expression was increased in neurons after mechanically injury, and the injured neurons uptook the astrocyte-secreted apoE, as well. Furthermore, the injured neurons stimulated astrocytes to express more apoE through the ERK signaling pathway. Mechanical injury triggered the neurons to increasingly synthesized apoE and uptook exogenous apoE, while stimulators released from injured neurons elevated astrocytes in apoE expression and secretion.


► The mechanical injured neurons increased apoE expression, but not secretion.
► The mechanical injured neurons uptook more apoE from external environment.
► Some injured neurons factors elevate apoE level of astrocytes, via ERK pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 515, Issue 1, 25 April 2012, Pages 77–81
نویسندگان
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