Accessory olfactory bulb neurons are required for maintenance but not induction of V2R vomeronasal receptor gene expression in vitro

► Effects of AOB neurons on V2R expression were examined using RT-PCR analysis. ► V2R expression declined over culture period without AOB neurons. ► But V2R expression was maintained by coculturing with AOB neurons. ► TTX-treatment resulted in a marked decrease in the V2R expression in the coculture. ► V2R expression is maintained by the active interaction with AOB neurons.