کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4346884 | 1296808 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Alterations in protein expression and membrane properties during Müller cell gliosis in a murine model of transient retinal ischemia
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Alterations in protein expression and membrane properties during Müller cell gliosis in a murine model of transient retinal ischemia Alterations in protein expression and membrane properties during Müller cell gliosis in a murine model of transient retinal ischemia](/preview/png/4346884.png)
چکیده انگلیسی
Retinal Müller glial cells are involved in K+ ion homeostasis of the tissue. Inwardly rectifying K+ (Kir) channels play a decisive role in the process of spatial K+ buffering. It has been demonstrated that Kir-mediated currents of Müller cells are downregulated in various cases of retinal neurodegeneration. However, this has not yet been verified for any murine animal model. The aim of the present study was to investigate Müller cells after transient retinal ischemia in mice. High intraocular pressure was applied for 1Â h; the retina was analysed 1 week later. We studied protein expression in the tissue by immunohistochemistry, and membrane currents of isolated cells by patch-clamp experiments. We found the typical indicators of reactive gliosis such as upregulation of glial fibrillary acidic protein. Moreover, the membrane capacitance of isolated Müller cells was increased and the amplitudes of Kir-mediated currents were slightly, but significantly decreased. This murine high intraocular pressure model of transient retinal ischemia is proposed as a versatile tool for further studies on Müller cell functions in retinal degeneration.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 472, Issue 1, 12 March 2010, Pages 73-78
Journal: Neuroscience Letters - Volume 472, Issue 1, 12 March 2010, Pages 73-78
نویسندگان
Petra G. Hirrlinger, Elke Ulbricht, Ianors Iandiev, Andreas Reichenbach, Thomas Pannicke,