کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4347514 1296845 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The identification of proteoglycan, collagen and neuron in precursor cells from human fetal spinal cord
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
The identification of proteoglycan, collagen and neuron in precursor cells from human fetal spinal cord
چکیده انگلیسی
After spinal cord injury (SCI), a loss of myelinating oligodendrocytes and neurons occurs. The functional recovery of injured spinal cords is the principal objective of SCI repair. Cell transplantation may prove beneficial to help replace lost myelin and spinal cord circuitry. In this study, we demonstrated that neural precursor cells (hNPCs) from human fetal spinal cord express three types of proteoglycan proteins-chondroitin sulfate, keratan sulfate, and cartilage proteoglycan (an extracellular matrix detected in normal spinal cord), and non-proteoglycan matrix collagen. Both proteoglycan and collagen evidenced profound immunoreactivity in double-stained cell clusters. In addition, whether or not hNPCs were capable of differentiating into a variety of cells, including GABAergic and cholinergic neurons, were assessed. The differentiated cells of eight passages grown on a monolayer expressed the human nuclear protein (HNu), the progenitor marker nestin, GAD, ChAT, TJU, and MAP-2. These results indicate that hNPCs may prove to be candidate cells for therapeutic SCI strategies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 457, Issue 3, 3 July 2009, Pages 151-154
نویسندگان
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