کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4348820 | 1296907 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Glutamate-induced retinal lipid and protein damage: The protective effects of catechin Glutamate-induced retinal lipid and protein damage: The protective effects of catechin](/preview/png/4348820.png)
Glutamate toxicity has been implicated in various retinal diseases. Green tea leaf extract catechin has protective effects against cellular toxicity. This study investigated the effects of catechin on the glutamate-treated retina. Porcine retinal homogenates were incubated with glutamate (20 nmol) at 37 °C for 60 min. Catechin was co-incubated with the glutamate-treated retina in the same condition. The malondialdehyde (MDA) levels were determined as an index of lipid peroxidation (LPO). Differential protein expressions were derived from two-dimensional gel electrophoresis. Mass spectrometry was conducted to identify the proteins. Glutamate increased the retinal MDA (p < 0.0001) and catechin reversed the effect (p < 0.0001). There were significant changes in seven proteins after the glutamate treatment (p < 0.05), namely, heterogeneous ribonucleoprotein, thioredoxin peroxidase, 5-hydroxytryptamine receptor, pyruvate dehydrogenase, ARHA protein, peroxiredoxin 6 and proteasome. Catechin significantly reversed the changes in thioredoxin peroxidase, 5-hydroxytryptamine receptor, peroxiredoxin 6 and pyruvate dehydrogenase (p < 0.05). Our study shows that (a) retinal glutamate toxicity is mediated by LPO and protein modification, and (b) catechin ameliorates the toxicity.
Journal: Neuroscience Letters - Volume 432, Issue 3, 27 February 2008, Pages 193–197