کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4349412 | 1296940 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Galectin-3 expression is correlated with abnormal prion protein accumulation in murine scrapie Galectin-3 expression is correlated with abnormal prion protein accumulation in murine scrapie](/preview/png/4349412.png)
To investigate the involvement of galectin-3 in the process of neurodegeneration in prion diseases, the expression and cellular localization of galectin-3 in the brain were studied in scrapie, a mouse model of prion disease. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analyses showed that the expression of galectin-3 protein and mRNA was induced in scrapie-affected brains, particularly at the time when the abnormal prion protein PrPSc began to accumulate in the brains. Immunohistochemically, immunostaining for galectin-3 was found mainly in B4-isolectin-positive cells (presumably activated microglia/macrophages), but not in astrocytes. Galectin-3 immunoreactivity was localized mainly in areas of PrPSc accumulation and neuronal death in scrapie-infected brains. These findings suggest that the expression of galectin-3 by activated microglia/macrophages in prion disease correlates with abnormal prion protein accumulation.
Journal: Neuroscience Letters - Volume 420, Issue 2, 13 June 2007, Pages 138–143