کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4349794 | 1296957 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Roles of NF-κB and MAPK signaling pathways in morphological and cytoskeletal responses of microglia to double-stranded RNA
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Following virus infection of the central nervous system, microglia become activated and undergo morphological as well as functional transformations, thereby initiating effective antiviral actions. Herein, we have examined the contribution of nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways to cell shape determination and cytoskeletal organization in microglia upon stimulation with double-stranded RNA (dsRNA), a conserved molecular pattern of virus infection. Under non-proliferative condition, microglial MG6-1 cells displayed a distinctive morphology with spinescent processes and small somata. Following dsRNA stimulation, the process-bearing microglial cells exhibited swift and drastic changes in cell morphology, filamentous actin (F-actin) structure, and intracellular signaling. In the dsRNA-stimulated microglial cells, the activation of c-Jun N-terminal kinase (JNK) pathway was involved in morphological alteration into an ameboid state. We also found that p38 signaling pathway negatively regulates the formation of cytoplasmic vacuoles in microglial cells. Furthermore, the dsRNA-induced accumulation of F-actin was partly mediated by NF-κB, JNK, and p38 pathways. These results indicate that NF-κB and MAPK signaling pathways mediate morphological and cytoskeletal changes during dsRNA-induced microglial activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 414, Issue 3, 13 March 2007, Pages 222-227
Journal: Neuroscience Letters - Volume 414, Issue 3, 13 March 2007, Pages 222-227
نویسندگان
Kazuo Nakamichi, Megumi Saiki, Hiroshi Kitani, Yuki Kuboyama, Kinjiro Morimoto, Mutsuyo Takayama-Ito, Ichiro Kurane,