کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4350138 1296974 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cullin 5 gene expression in the rat cerebral cortex and hippocampus following traumatic brain injury (TBI)
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Cullin 5 gene expression in the rat cerebral cortex and hippocampus following traumatic brain injury (TBI)
چکیده انگلیسی

Cullin-5 (Cul-5), a member of the cullin gene family of scaffold proteins of E3 ubiquitin-ligase complexes, has a role in proteolysis and cell cycle regulation. We recently demonstrated that cul-5 mRNA is ubiquitously expressed in the central nervous system. The present study used quantitative real time polymerase chain reaction and western blotting to measure changes in cul-5 mRNA and Cul-5 protein expression, respectively, in the injured CNS in response to traumatic brain injury (TBI). cul-5 mRNA levels were significantly decreased in the ipsilateral rat cerebral cortex on Days 1 and 7, but not on Day 3 following TBI. In the ipsilateral hippocampus, cul-5 mRNA was significantly reduced on Day 1 after TBI. Cul-5 protein levels were significantly decreased in the ipsilateral rat cerebral cortex on Days 1–7 post-TBI while levels were significantly lower in the ipsilateral hippocampus on Days 3–7 post-TBI. Since Cul-5 is ubiquitously expressed in eukaryotic cells and is linked to proteasome-mediated protein degradation, it may have a role in CNS cell fate determination under conditions of traumatic stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 409, Issue 1, 27 November 2006, Pages 65–69
نویسندگان
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