کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4350402 | 1615188 | 2006 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Microtubule-bundling activity of APC is stimulated by interaction with PSD-95 Microtubule-bundling activity of APC is stimulated by interaction with PSD-95](/preview/png/4350402.png)
Adenomatous polyposis coli (APC) tumor suppressor protein binds to microtubules, leading to microtubule bundling and stabilization. The protein also interacts with postsynaptic density (PSD)-95, a major scaffolding protein in neurons. Here, we analyzed the effects of PSD-95 on the microtubule-bundling activity of APC. The coexpression of APC and PSD-95 in COS-7 cells enhanced microtubule-bundle formation compared with the expression of APC alone. A mutant APC variant that does not associate with PSD-95 did not enhance microtubule bundling, despite coexpression with PSD-95. Immunoelectron microscopy showed that the APC–PSD-95 complex sometimes colocalized on microtubules in processes of cultured neurons. These results suggest that the microtubule-bundling activity of APC is regulated by its interaction with PSD-95, which might modulate microtubule architecture and dynamics in neurons.
Journal: Neuroscience Letters - Volume 403, Issues 1–2, 31 July 2006, Pages 68–72