Parallel high throughput neuronal toxicity assays demonstrate uncoupling between loss of mitochondrial membrane potential and neuronal damage in a model of HIV-induced neurodegeneration

► We have further defined our in vitro model of HIV-induced neurotoxicity. ► Loss of neuronal marker, MAP2, begins as early as 2 h. ► MAP2 loss is preceded by a loss of mitochondrial membrane potential (Δψm). ► Inhibitors of calpains, blocked MAP2 loss, but did not prevented the loss of Δψm.