کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4352316 | 1298105 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellular distribution of the nicotinic acetylcholine receptor α7 subunit in rat hippocampus
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کلمات کلیدی
PBSbis(sulfosuccinimidyl) suberateα-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptorMLKAHSPBGTbungarotoxinBSSTBSTα-bungarotoxinOHSCBS3RIPAaCSFPFAAMPARnAChRHRPPVDFINSBSA - BSADMSO - DMSObovine serum albumin - آلبومین سرم گاوStreptavidin - استرپتاویدینSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتinsulin - انسولینSerum-free - بدون سرمHippocampal slice - برش هیپوکامپstandard error of the mean - خطای استاندارد میانگینDIV - دیوDimethyl sulfoxide - دیمتیل سولفواکسیدdays in vitro - روز in vitroradioimmunoprecipitation assay - سنجش radioimmunoprecipitationOrganotypic culture - فرهنگ ارگانیکorganotypic hippocampal slice culture - فرهنگ لغت ارگ تیپیک هیپوکامپPhosphate buffered saline - فسفات بافر شورTrafficking - قاچاقGenistein - ماده جنستئینartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیbalanced salt solution - محلول نمک متعادلSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیparaformaldehyde - پارافرمالدهیدHorseradish peroxidase - پراکسیداز هوررادیشPolyvinylidene fluoride - پلی وینیلیدین فلورایدSkim milk powder - پودر شیر بدون چربیGABAAR - گابارnicotinic acetylcholine receptor - گیرنده استیلکولین نیکوتین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
The hippocampus is a region of the mammalian brain that has been extensively studied due to its role in many forms of memory. To better understand hippocampal function, significant attention has focused upon the cellular distribution of ligand-gated ion channels. Despite strong cholinergic innervation from the basal forebrain and a dense expression of nicotinic acetylchoine receptors (nAChRs), the cellular distribution of subunits forming these receptors has received little attention. We used organotypic hippocampal slice cultures (OHSCs) to study native α7 subunits, which, unlike other nAChR subunits, form a homomeric receptor. Cell-surface biotinylation, cross-linking of surface proteins, and sub-cellular fractionation all revealed a very limited presence of the subunit at the plasma membrane. In contrast, subunits of other receptors displayed significant surface expression. Notably, subunits in adult hippocampal tissue were distributed in a fashion similar to that observed in OHSCs. To monitor α7 subunits contained in functional nAChRs, a colourimetric assay using α-bungarotoxin (a specific α7 nAChR antagonist) was developed, and revealed a majority of binding at the cell surface. To change α7 subunit distribution, OHSCs were treated with compounds known to affect other ionotropic receptors-insulin, genistein, and elevated external K+; however, neither subunit surface expression nor antagonist binding was affected. Our data reveal that hippocampal neurons possess a large internal population of α7 subunits under basal conditions, which persists during stimuli affecting tyrosine phosphorylation or neuronal activity. The nature of the internal pool of α7 subunits remains to be determined, but should have important implications for hippocampal activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Research - Volume 65, Issue 3, November 2009, Pages 296-306
Journal: Neuroscience Research - Volume 65, Issue 3, November 2009, Pages 296-306
نویسندگان
John G. Mielke, Geoffrey A.R. Mealing,