کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4358611 | 1300444 | 2013 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of the metabolic pathway involved in assimilation of acenaphthene in Acinetobacter sp. strain AGAT-W Characterization of the metabolic pathway involved in assimilation of acenaphthene in Acinetobacter sp. strain AGAT-W](/preview/png/4358611.png)
Utilization of an enrichment technique led to isolation of a bacterium from municipal waste-contaminated soil in which acenaphthene was used as the sole source of carbon and energy. The isolate was identified as Acinetobacter sp. strain AGAT-W based on morphological, nutritional and biochemical characteristics and 16S rRNA sequence analysis. Characterization of metabolites by HPLC and GC–MS suggested hydroxylation of acenaphthene to 1-acenaphthenol, which was subsequently transformed to catechol via acenaphthenequinone, naphthalene-1,8-dicarboxylic acid, 1-naphthoic acid and salicylic acid before entering into the tricarboxylic acid cycle. Detection of key enzymes, viz., 1-acenaphthenol dehydrogenase, salicylaldehyde dehydrogenase and catechol 1,2-dioxygenase, in the cell-free extract of Acinetobacter sp. further supported the proposed degradation pathway. This study proposes a metabolic pathway involved in acenaphthene assimilation in strain AGAT-W.
Journal: Research in Microbiology - Volume 164, Issue 2, February–March 2013, Pages 155–163