Antibacterial activity of class IIa bacteriocin Cbn BM1 depends on the physiological state of the target bacteria

Carnobacteriocin BM1 (Cbn BM1) is a class IIa bacteriocin produced by Carnobacterium maltaromaticum CP5 isolated from a French mold ripened cheese. Numerous studies highlight variations in numerous parameters, such as bacterial membrane composition and potential, according to physiological changes. In this work, the mechanism of action of an oxidized form of Cbn BM1 was studied on C. maltaromaticum DSM20730 in log and stationary growth phases. Membrane integrity assessment and high resolution imaging by atomic force microscopy confirmed the link between physiological state and bacterial sensitivity to Cbn BM1. Indeed, these approaches enable visualizing morphological damage of C. maltaromaticum DSM20730 only in an active dividing state. To specifically address the interaction between peptide and bacterial membrane, fluorescence anisotropy measurements were conducted. Results revealed strong modifications in membrane fluidity by Cbn BM1 only for C. maltaromaticum DSM20730 in log growth phase. In a similar way, the Δψ component, but not the ΔpH component of the proton-motive force, was perturbed only for bacteria in log growth phase. These results clearly show that a class IIa bacteriocin antimicrobial mechanism of action can be modulated by the physiological state of its target bacteria.