کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4358824 1300460 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Construction of isogenic mutants in Streptococcus gallolyticus based on the development of new mobilizable vectors
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Construction of isogenic mutants in Streptococcus gallolyticus based on the development of new mobilizable vectors
چکیده انگلیسی

Streptococcus gallolyticus is an emerging cause of infective endocarditis that has been epidemiologically linked to colorectal cancer. S. gallolyticus is poorly transformable using electroporation and no defined mutant has been published yet. Hence, we used mobilization to introduce plasmid DNA from Streptococcus agalactiae into S. gallolyticus using the transfer origin of the conjugative element TnGBS1 (oriTTnGBS1), followed by a classical homologous recombination technique. Two isogenic mutants of S. gallolyticus UCN34, one deleted for the pil1 pilus operon and another for the sortase A gene, were constructed and characterized. This genetic tool should help in unravelling virulence mechanisms of this bacterium.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 164, Issue 10, December 2013, Pages 973–978
نویسندگان
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