کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4358980 1300474 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The photosynthetic membrane proteome of Rhodobacter sphaeroides R-26.1 exposed to cobalt
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
The photosynthetic membrane proteome of Rhodobacter sphaeroides R-26.1 exposed to cobalt
چکیده انگلیسی
Cells of the carotenoidless strain R-26.1 of Rhodobacter sphaeroides were grown in the presence of a high concentration (5 mM) of cobalt ions. The photosynthetic intracytoplasmic membranes were isolated and investigated by proteomic analysis using non-denaturating blue native electrophoresis in combination with LC-ESI-MS/MS. Comparison with intracytoplasmic membranes of cells grown under control conditions showed a change in the relative amount of proteins belonging to the photosynthetic apparatus, with net downregulation of light-harvesting complexes and increased concentration of the nude reaction center (RC), as well as upregulation of enzymes related to chemoorganotrophy. These effects represent possible bacterial adaptation so as to retrieve energy for metabolic processes from sources alternative to less efficient photosynthesis. The influence of cobalt on the photochemistry of the RC in cell extracts was also investigated by charge recombination. The kinetics of the charge recombination reaction was found to be slower in extracts from cells exposed to Co2+, indicating that the reorganization of the photosynthetic apparatus also involves its photochemical core.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 162, Issue 5, June 2011, Pages 520-527
نویسندگان
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