کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4366571 1616576 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Control of pathogens in biofilms on the surface of stainless steel by levulinic acid plus sodium dodecyl sulfate
ترجمه فارسی عنوان
کنترل پاتوژن ها در بیوفیلم ها بر روی سطح فولاد ضد زنگ توسط اسید لووولینیک به همراه سدیم دودسیل سولفات
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


• Efficacy of commonly used sanitizers to inactivate cells in biofilms was evaluated.
• 3% LVA + 2% SDS achieved > 6.9-log CFU reduction of the pathogens in biofilms.
• Integrity of cell envelopes was damaged by levulinic acid plus SDS.

The efficacy of levulinic acid (LVA) plus sodium dodecyl sulfate (SDS) to remove or inactivate Listeria monocytogenes, Salmonella Typhimurium, and Shiga toxin-producing Escherichia coli (STEC) in biofilms on the surface of stainless steel coupons was evaluated. Five- or six-strain mixtures (ca. 9.0 log CFU/ml) of the three pathogens were separately inoculated on stainless steel coupons. After incubation at 21 °C for 72 h, the coupons were treated for 10 min by different concentrations of LVA plus SDS (0.5% LVA + 0.05% SDS, 1% LVA + 0.1% SDS, and 3% LVA + 2% SDS) and other commonly used sanitizers, including a commercial quaternary ammonium-based sanitizer (150 ppm), lactic acid (3%), sodium hypochlorite (100 ppm), and hydrogen peroxide (2%). The pathogens grew in the biofilms to ca. 8.6 to 9.3 log CFU/coupon after 72 h of incubation. The combined activity of LVA with SDS was bactericidal in biofilms for cells of the three pathogens evaluated, with the highest concentrations (3% LVA + 2% SDS) providing the greatest log reduction. Microscopic images indicated that the cells were detached from the biofilm matrix and the integrity of cell envelopes were decreased after the treatment of LVA plus SDS. This study is conducive to better understanding the antimicrobial behavior of LVA plus SDS to the foodborne pathogens within biofilms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Food Microbiology - Volume 207, 17 August 2015, Pages 1–7
نویسندگان
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