Role of RpoS in the susceptibility of low salinity-adapted Vibrio vulnificus to environmental stresses

Vibrio vulnificus is an opportunistic pathogen commonly found in oyster and marine environments, which frequently encounters low salinity stress in its natural and food processing environment. In this study, the responses of a V. vulnificus wild-type strain C78140o and its rpoS isogenic mutant AH1 to sublethal low salinity were examined to investigate the role of rpoS in this response. Both strains, adapted in low salinity (0.4% NaCl), were protected against the lethal low salinity (0.1% NaCl), but were not protected against heat (45 °C) or acid stress (pH 3.5), and were sensitized against 5% bile. Protection of the adapted cells against the lethal low salinity was not inhibited by the addition of chloramphenicol. Hemolysis was detected only in the adapted C78140o cells and its spent medium, and was inhibited by chloramphenicol. Transcription of the mechanosensitive channels (VVl_1542 and VVl_2579) and an aquaporin gene (VVl_2010) was markedly increased in the wild-type cells adapted in low salinity medium, while transcription of these genes was slightly enhanced or inhibited in AH1 cells. Results of this study support the active role of rpoS in the low salinity adaptation of V. vulnificus by regulating the expression of virulence and low salinity-associated factors, although rpoS is not related to the immediate protection of the adapted cells against lethal low salinity.