کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4371797 | 1302541 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Trypanosoma brucei AMP-activated kinase subunit homologs influence surface molecule expression
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The African trypanosome, Trypanosoma brucei, can gauge its environment by sensing nutrient availability. For example, procyclic form (PF) trypanosomes monitor changes in glucose levels to regulate surface molecule expression, which is important for survival in the tsetse fly vector. The molecular connection between glycolysis and surface molecule expression is unknown. Here we partially characterize T. brucei homologs of the β and γ subunits of the AMP-activated protein kinase (AMPK), and determine their roles in regulating surface molecule expression. Using flow cytometry and mass spectrometry, we found that TbAMPKβ or TbAMPKγ-deficient parasites express both of the major surface molecules, EP- and GPEET-procyclin, with the latter being a form that is expressed when glucose is low such as in the tsetse fly. Last, we have found that the putative scaffold component of the complex, TbAMPKβ, fractionates with organellar components and colocalizes in part with a glycosomal marker as well as the flagellum of PF parasites.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 123, Issue 3, November 2009, Pages 250-257
Journal: Experimental Parasitology - Volume 123, Issue 3, November 2009, Pages 250-257
نویسندگان
Clarice S. Clemmens, Meredith T. Morris, Todd A. Lyda, Alvaro Acosta-Serrano, James C. Morris,