کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4372260 | 1302598 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Plasmodium falciparum: Biochemical characterization of the cysteine protease falcipain-2â²
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DTTGSHAmCE-647-amino-4-methyl coumarin - 7 آمینو 4-متیل کومارینDMSO - DMSOtrans-Epoxysuccinyl-l-leucylamido-(4-guanidino)butane - ترانس اپوکسی سوزن سیلین ال-لوسیلامیدو (4-گانیدینو) بوتانdithiothreitol - دیتیوتریتولDimethyl sulfoxide - دیمتیل سولفواکسیدCysteine protease - سیستئین پروتئازFalcipain - فالسیپینMalaria - مالاریاHemoglobin - هموگلوبینProteinase - پروتئینازPlasmodium falciparum - پلاسمودیوم فالسیپارومreduced glutathione - کاهش گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Plasmodium falciparum: Biochemical characterization of the cysteine protease falcipain-2â² Plasmodium falciparum: Biochemical characterization of the cysteine protease falcipain-2â²](/preview/png/4372260.png)
چکیده انگلیسی
The Plasmodium falciparum cysteine proteases falcipain-2 and falcipain-3 are hemoglobinases and potential antimalarial drug targets. The falcipain-2â² gene was identified recently and is nearly identical in sequence to falcipain-2. The product of this gene has not been studied previously. The mature protease domain of falcipain-2â² was expressed in Escherichia coli, purified, and refolded to active enzyme. Functional analysis revealed similar biochemical properties to those of falcipain-2, including pH optima (pH 5.5-7.0), reducing requirements, and substrate preference. Studies with cysteine protease inhibitors showed similar inhibition of falcipain-2 and falcipain-2â², although specificities were not identical. Considering activity against the presumed biological substrate, both enzymes readily hydrolyzed hemoglobin. Our results confirm that falcipain-2â² is an active hemoglobinase and suggest that falcipain-2 and falcipain-2â² play similar roles in erythrocytic parasites but that, for promising cysteine protease inhibitors, it will be important to confirm activity against this additional target.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Parasitology - Volume 112, Issue 3, March 2006, Pages 187-192
Journal: Experimental Parasitology - Volume 112, Issue 3, March 2006, Pages 187-192
نویسندگان
Naresh Singh, Puran S. Sijwali, Kailash C. Pandey, Philip J. Rosenthal,