کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4408848 1618867 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Estrogen receptor affinity chromatography: A new method for characterization of novel estrogenic disinfection by-products
ترجمه فارسی عنوان
کروماتوگرافی جذب گیرنده استروژن: یک روش جدید برای توصیف محصولات ضد عفونی جدید استروژنیک است
کلمات کلیدی
غربالگری استروژن، کروماتوگرافی وابستگی، ضد عفونی به وسیله محصول، استروژن، دی اتیلستیل بسترول
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
چکیده انگلیسی


• A novel affinity chromatography was developed to screen estrogenic compounds.
• HPLC peak areas can be used to determine the relative receptor binding affinity.
• Unknown estrogenic DBPs of chlorination DES were isolated and identified.

To identify the unknown estrogenic disinfection by-products (DBPs) from the chlorination extract, an effective method based on affinity chromatography with immobilized human recombinant estrogen receptor α (ERα) was developed, which has an advantage in targeting different potential estrogenic compounds from mixed sample simultaneously by comparing their relative binding activities to ER. The new method worked well for six known environmental estrogens. To further test the validity of this method for unknown chemicals, six DBPs of diethylstilbestrol (DES) with relatively strong ER binding affinity after chlorination were isolated and identified. It was found that except for 2-chloro-DES which showed 1.36 times stronger binding affinity than DES, most of the by-products bound to ER much more weakly than DES. All these seven by-products induced a dose-dependent transcriptional activation in two-hybrid-yeast assays. Z,Z-dienestrol (DE) and 2-chloro-DES, which exhibiting the weakest and the strongest binding affinity, were further tested for their transcriptional potential as 0.00243 and 0.014 compared to DES, respectively. However, they were still potential harmful environmental estrogenic disruptors as their estrogenic activities were much stronger than that of bisphenol A (BPA). These results demonstrated that the new method can help to screen unknown estrogenic compounds from mixture more efficiently.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 104, June 2014, Pages 251–257
نویسندگان
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