کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4409869 1307514 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
PCR–ELISA detection of estrogen receptor β mRNA expression and plasma vitellogenin induction in juvenile sole (Solea solea) exposed to waterborne 4-nonylphenol
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
PCR–ELISA detection of estrogen receptor β mRNA expression and plasma vitellogenin induction in juvenile sole (Solea solea) exposed to waterborne 4-nonylphenol
چکیده انگلیسی

In this study, the effect of 4-nonylphenol (4NP) on reproductive axis of sole (Solea solea) has been investigated by using selected biomarkers of estrogenic effects: vitellogenin (VTG) and estrogen receptor β (ERβ) mRNA. Furthermore, an enzyme linked immunosorbent assay (ELISA) detection system of reverse transcriptase-polymerase chain reaction (RT-PCR) products for the analysis of sole ERβ mRNA expression was developed and validated. The proposed method allows large-scale analyses of different mRNAs in fast and not expensive way. Our results have demonstrated that the PCR–ELISA method developed shows high sensitivity, good reproducibility and also the potential for semi-quantitative analysis of hepatic ERβ mRNAs. Both plasma VTG level and ERβ mRNA expression were increased in tested animals following a short exposure to environmental relevant concentrations (10−6 M) of 4NP, confirming the functional role of ERβ in the regulation of xenoestrogens-induced production of VTG. The methodology provided in the present study together with the preliminary results on the hepatic expression of ERβ may be useful in environmental xenoestrogens monitoring studies, using flatfish as “sentinel” species.


► An ELISA detection system of RT-PCR products for analyzing ERβ mRNA is proposed.
► The method allows coamplification of different mRNAs in fast and not expensive way.
► Juvenile sole exposed to 4NP showed high levels of both VTG and ERβ mRNA.
► Hepatic ERβ plays a functional role in the regulation of 4NP-induced VTG synthesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 86, Issue 9, March 2012, Pages 919–925
نویسندگان
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