کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4410161 1307532 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reduction of U(VI) by the deep subsurface bacterium, Thermus scotoductus SA-01, and the involvement of the ABC transporter protein
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Reduction of U(VI) by the deep subsurface bacterium, Thermus scotoductus SA-01, and the involvement of the ABC transporter protein
چکیده انگلیسی

In this study we investigated the effect of uranium on the growth of the bacterium Thermus scotoductus strain SA-01 as well as the whole cell U(VI) reduction capabilities of the organism. Also, site-directed mutagenesis confirmed the identity of a protein capable of a possible alternative mechanism of U(VI) reduction. SA-01 can grow aerobically in up to 1.25 mM uranium and has the capability to reduce low levels of U(VI) in under 20 h. TEM analysis performed on cells exposed to uranium showed extracellular and membrane-bound accumulation of uranium. The reductase-like protein was surprisingly identified as a peptide ABC transporter, peptide-binding protein. This study showcases the concept of protein promiscuity, where this protein with a distinct function in situ can also have the unintended function of a reactant for the reduction of U(VI).


► We grew Thermusscotoductus SA-01 aerobically in up to 1.25 mM uranium.
► Using whole cells SA-01 reduced low levels of U(VI) in under 20 h with various electron donors.
► TEM analysis showed extracellular and membrane-bound accumulation of uranium.
► A peptide ABC transporter, peptide-binding protein is shown to be capable of U(VI) interactions.
► A possible mechanism for uranium(VI) reduction by the protein is elucidated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 86, Issue 6, February 2012, Pages 572–577
نویسندگان
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