Characterization of dissolved organic matter using high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) with a multiple wavelength absorbance detector

► Error in MW evaluation can be demonstrated by HPLC-SEC with multiple wavelength absorbance detection. ► Some additional properties information of DOM can provide HPLC-SEC with multiple wavelength absorbance detection. ► Apparent MW of DOM can be predicted by spectroscopic parameter of HPLC-SEC with multiple wavelength absorbance detection.