A freshwater amphipod toxicity test based on postexposure feeding and the population consumption inhibitory concentration

Consumption inhibition in natural populations, namely due to contaminants, may have direct and immediate effects on ecosystems, by hampering ecosystem key functions (e.g., organic matter decomposition, grazing), long before effects at the individual level (e.g., reproduction, growth, emergence) have time-delayed consequences at successively higher levels of biological organization. The present study aimed at developing a cost-effective (short and easy) toxicity test based on the postexposure feeding of a freshwater amphipod present in the Iberian Peninsula and at evaluating the immediate impact of contamination on the population-level consumption rate. First, methodologies to quantify postexposure feeding were developed and optimized, the most important criterion being a feeding period as short as possible to minimize physiological recovery from the contaminant exposure. Second, the sensitivities of 48-h postexposure feeding and 48-h lethality tests were compared, using a reference chemical – copper. Third, the latter responses were integrated in a single parameter, the median population consumption inhibitory concentration. When Echinogammarus meridionalis males were fed on 100 defrosted Artemia franciscananauplii during 30 min in darkness at 19–21 °C, the eaten proportion was approximately 80%, without truncated data distributions. The 48-h median effective copper concentration for postexposure feeding was approximately two times lower than the 48-h LC50 – 91 versus 198 μg L−1, respectively. Two techniques were used to quantify the median population consumption inhibitory copper concentration, both leading to similar values (75 and 68 μg L−1). In conclusion, when contaminant concentrations elicit both lethality and feeding depression, the integration of both responses can provide an ecologically relevant evaluation on the potency of a contaminant to immediately disrupt ecosystem functioning.

► A short sublethal toxicity test with an Iberian freshwater amphipod was developed.
► Amphipod feeding rates on brine shrimp nauplii were easily and precisely quantified.
► Both feeding and survival potentially impact ecosystem functioning.
► The median population consumption inhibitory concentration of copper was ∼0.07 mg L−1.