Measurement of pyrene in the gills of exposed fish using synchronous fluorescence spectroscopy

A synchronous fluorescence spectroscopy (SFS) method was developed for determination of pyrene in the gills of exposed fish. The wavelength differences (Δλ) of 50 nm was maintained between excitation and emission wavelengths and it was found to be suitable for the effective determination of pyrene in fish gills; the peak were observed at λex 334.5 nm. Linear relationships between SFS intensity and the concentration of pyrene in n-hexane solution were established. It was demonstrated that the SFS method was effective, simple, and less expensive, providing an attractive alternative for the rapid analysis of pyrene in fish gills.

► Pyrene in gills was determined using synchronous fluorescence spectroscopy (SFS).
► The wavelength differences (Δλ) of 50 nm was found to be suitable for SFS method.
► The peaks were observed at λex 334.5 nm.
► The SFS method used in this paper is simple, effective, and less expensive.