کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4411682 1307604 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Testing of the mutagenicity and genotoxicity of metolcarb by using both Ames/Salmonella and Allium test
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Testing of the mutagenicity and genotoxicity of metolcarb by using both Ames/Salmonella and Allium test
چکیده انگلیسی

Mutagenic and genotoxic effects of metolcarb were investigated by both bacterial reverse mutation assay in Salmonella typhimurium TA97, TA98, TA100 and TA102 strains with or without metabolic activation system (S9) and Allium cepa root meristematic cells, respectively. Metolcarb was dissolved in DMSO in Ames/Salmonella test system. 0.1, 1 and 10 μg/plate doses of metolcarb were found to be mutagenic S. typhimurium TA98 without S9. In Allium root growth inhibition test, EC50 value was determined 200 ppm and 0.5xEC50, EC50 and 2xEC50 concentrations of metolcarb were introduced to onion tuber roots and distilled water used as a negative control. Mitotic index (MI), increased in all concentrations compared to control at each exposure time. While disturbed anaphase–telophase, chromosome laggards, stickiness and bridges were observed in anaphase–telophase cells, pro-metaphase, C-mitosis, polyploidy, binuclear cells and disturbed nucleus were observed in other cells. The results were also analyzed statistically by using SPSS for Windows, Mann–Whitney test and Duncan’s multiple range tests were performed respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 80, Issue 9, August 2010, Pages 1056–1061
نویسندگان
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