کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4411945 1307615 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of ivermectin on the liver of gilthead sea bream Sparus aurata: A proteomic approach
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Effect of ivermectin on the liver of gilthead sea bream Sparus aurata: A proteomic approach
چکیده انگلیسی

Gilthead sea bream Sparus aurata is the most commercialized Mediterranean aquacultured fish species. Ivermectin has recently (experimentally) started to be used to control ectoparasitic infestations in Mediterranean cultured marine fish. The potential hepatotoxicity of ivermectin was investigated in gilthead sea bream juveniles (35 g) following oral administration at the recommended dose of 0.2 mg kg−1 fish for 10 d. Difference Gel Electrophoresis Technology (DIGE) was used to study the effect of this treatment in gilthead sea bream liver protein profile under routine culture conditions. The 2D-DIGE protein maps obtained were analyzed using the DeCyder 6.5 software. The results obtained showed significant changes in the expression of 36 proteins respect to the control group. Among these proteins, six increased in abundance, and 30 decreased. Spot showing differential expression respect to the control were analyzed by mass spectrometry and database search, which resulted in three positive identifications corresponding to hepatic proteins involved in lipid metabolism (apoA-I), oxidative stress responses and energy generation (beta-globin, ATP synthase subunit beta). These proteins have not been previously associated to invermectin effect.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 80, Issue 5, July 2010, Pages 570–577
نویسندگان
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