کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4412510 1307641 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of square wave voltammetry method for the assessment of organophosphorus compound impact on the cholinesterase of pheretima with 2,6-dichloroindophenol as a redox indicator
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Development of square wave voltammetry method for the assessment of organophosphorus compound impact on the cholinesterase of pheretima with 2,6-dichloroindophenol as a redox indicator
چکیده انگلیسی

A square wave voltammetry method was developed for the assessment of organophosphorus (OPs) compound impact on the cholinesterase of pheretima with 2,6-dichloroindophenol (2,6-DCIP) as a redox indicator. The substrate of acetylthiocholine is hydrolysed by the cholinesterase (ChE) from soil animal pheretima, and the produced thiocholine reacts with the 2,6-DCIP to give obvious shift of electrochemical signal. The inhibition of ChE was assessed by measuring the enzyme activity before and after incubating with parathion-methyl. The reduction peak current of 2,6-DCIP decreases with the time of enzymatical reaction. The ChE loses almost 32.74% activity after 10 min incubation with 1 ng mL−1 paraoxon and 54.62% with 10 μg mL−1 paraoxon, while the activity that corresponds to 100 μg mL−1 paraoxon was nearly completely inhibited. This method can be employed to assess the inhibition of ChE and investigate OPs impact on environmental animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 77, Issue 1, September 2009, Pages 129–132
نویسندگان
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