کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4413352 | 1307674 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acute exposure of the aquatic macrophyte Callitriche obtusangula to the herbicide oxadiazon: The protective role of N-acetylcysteine
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Acute exposure of the aquatic macrophyte Callitriche obtusangula to the herbicide oxadiazon: The protective role of N-acetylcysteine Acute exposure of the aquatic macrophyte Callitriche obtusangula to the herbicide oxadiazon: The protective role of N-acetylcysteine](/preview/png/4413352.png)
چکیده انگلیسی
In this study we investigated the acute exposure of the aquatic macrophyte Callitriche obtusangula to the herbicide oxadiazon (Ronstar®). The toxic effects on C. obtusangula were evaluated, 24 h after exposure, by assessing visible necrotic leaf lesions and, 12 h after exposure, via analyses of dead cells and hydrogen peroxide (H2O2) deposits localized by histocytochemical analysis with Trypan blue and 3,3â²-diaminobenzidine (DAB), respectively. As a result, we found that 0.1275 μg Lâ1 a.i. (active ingredient) oxadiazon was the maximum concentration that produced no observable adverse effects (NOAEC) both at leaf and tissue levels, at any considered exposure time. Additionally, we assayed the protective effect of pre-treatment with 0.25 mM N-acetylcysteine (NAC), a cysteine donor, on the damage caused by the toxic herbicidal dose of 6.37 μg Lâ1 a.i to C. obtusangula, correlating the NAC observed protection to the direct H2O2-scavenging and to the enhancement of glutathione parameters. NAC-treated plants showed a fourfold increase in the GSH (reduced glutathione) + GSSG (oxidised glutathione) content (149.2 nmol gâ1 FW) compared to controls (36.1 nmol gâ1 FW); in the NAC + oxadiazon treatments, the GSH + GSSG content was more than fivefold higher (202.1 nmol gâ1 FW). GSH showed a similar trend in NAC and NAC + oxadiazon treatments, being six- (130.0 nmol gâ1 FW) and eightfold (185.0 nmol gâ1 FW) higher, respectively, compared to controls (20.7 nmol gâ1 FW). Accordingly, the GSH/GSSG ratio in NAC- and NAC + oxadiazon-treated plants was significantly increased compared to controls, indicating alleviation of oxidative stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 74, Issue 9, March 2009, Pages 1231-1237
Journal: Chemosphere - Volume 74, Issue 9, March 2009, Pages 1231-1237
نویسندگان
Marcello Iriti, Giulia Castorina, Valentina Picchi, Franco Faoro, Stefano Gomarasca,