Vitellogenin as a biomarker of exposure to estrogenic compounds in aquatic invertebrates: A review

Vitellogenins (Vg) are the major precursor of the egg-yolk proteins, vitellins (Vn), which provide energy reserves for embryonic development in oviparous organisms. In mature females, Vg are generally synthesised in response to endogenous estrogens, such as 17β-estradiol (E2), released into the bloodstream and then stored in developing oocytes. In males, the Vg gene, although present, is normally silent. However, it may be activated by (xeno)-estrogens. These substances belong to a large and heterogeneous group of environmental contaminants capable of altering endocrine functions in organisms. For this reason, they are named endocrine disrupting chemicals (EDCs). Natural steroidal estrogens [E2, estrone (E1), estriol (E3)] and synthetic chemicals, such as pesticides, polychlorinated biphenyls (PCBs) and household products (surfactants) are estrogenic compounds widespread in aquatic ecosystems. The main sources of contamination mostly stem (or originate) from municipal and industrial effluents, agricultural practices, livestock wastes and sewage treatment plants (STP). Recently, Vg induction, mainly in males and immature females, has been proposed as a useful biomarker to assess estrogenic contamination in aquatic environments. Indeed, estrogenicity of individual chemicals and mixtures has extensively been evaluated, in both laboratory and field studies, albeit mostly in fish. In contrast, limited attention has centred on evaluating xenoestrogen effects in aquatic invertebrates, probably owing to limited knowledge of their endocrinology. This review focuses on a brief description of xenoestrogens and their occurrence in aquatic environments, and on Vg induction in aquatic invertebrates in response to both experimental and environmental exposure to estrogenic compounds. Results of recent field and laboratory studies are presented. In addition, new perspectives about the use of Vg induction as a biomarker of exposure to estrogenic compounds are also discussed.