Comparison of a bioremediation process of PAHs in a PAH-contaminated soil at field and laboratory scales

A laboratory experiment was carried on the same initial soil and at the same time than a windrow treatment in order to compare results at field and laboratory scales for a soil mainly contaminated with PAHs. After 6 months, laboratory experiments gave similar but less scattered results than those obtained in the field indicating that the field biotreatment was well optimised. The total amount of PAHs degraded after 6 months was ca. 90% and degradation rates followed a negative exponential trend. Relative degradation rates of 3- and 4-ring PAHs were about 32 and 7.2 times greater than those of 5- and 6-ring PAHs, respectively. With respect to the bacterial community, bacteria belonging to Gamma-proteobacteria persisted whereas Beta-proteobacteria appeared after three months of biotreatment when PAH concentration was low enough to render the soil non-ecotoxic.

► Laboratory experiment representative of PAH-contaminated soil biotreatment.
► Beta-proteobacteria : a good bioindicator to estimate the endpoint of biotreatment.
► Optimizing biotreatment conditions with a laboratory experiment.
► Monitoring biotreatment process with a laboratory experiment.