Mature non-native black-locust (Robinia pseudoacacia L.) forest does not regain the lichen diversity of the natural forest

The responses of lichens to habitat changes caused by invasive trees are poorly understood. Invasive forest trees may impact epiphytic lichens by altering both substrate and stand conditions.Previous research has demonstrated that black locust invasion, associated with intensive exploitation of native oak forests, led to dramatic shifts in lichen composition. However, it is not clear if, along with stand aging, black locust formations regain forest species. The main aim of this study was to test whether the succession of black locust stands promotes a lichen succession leading to assemblages in mature black locust stands which are similar to those of native forests. To test the influence of macro-environmental conditions, we performed the study in two bioclimatically different areas of Italy. The epiphytic lichen biota of native oak and chestnut stands was compared with that of black locust stands of different successional stages. In both regions we did not find a lichen succession in black locust stands of different age, and mature black-locust stands did not recover the diversity of epiphytic species, which are lost by the replacement of the native forests by black locust. The absence of this pattern may be caused by factors related to the management of black locust stands, and to bark features. The different bioclimatic conditions between the two study areas may explain differences in the lichen biota of native forests, while that of black locust stands tend to be similar between regions, suggesting that forest habitat changes associated with the spread of black locust could decrease lichen diversity among bioclimatically different regions.

► We did not find a lichen succession in non-native black locust stands of different age.
► Non-native black locust stands do not recover lichen diversity of native forests.
► Black locust stands mainly host nitrogen tolerant lichens.
► Black locust stands host similar lichen communities in bioclimatically diverse regions.