کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4430 226 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of ricinoleic acid estolide with free and immobilized lipase from Candida rugosa
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Production of ricinoleic acid estolide with free and immobilized lipase from Candida rugosa
چکیده انگلیسی

Ricinoleic acid estolide was produced by using free and immobilized Candida rugosa lipase at moderate temperature in a bioreactor. This work describes the immobilization of C. rugosa lipase on 10 different supports by covalent binding and physical adsorption, and how of the most suitable immobilized derivative was selected. The comparison was mainly based on the enzyme content and on the activity results. An anion exchange resin was judged to be the most appropriate support and the corresponding immobilization process was investigated and optimized. Although repeated batch reactions using the same derivative are not entirely advisable, the reaction proceeds at a noticeably slower rate and the degree of condensation reached is lower when the same amount of protein as in the derivative is added to the bioreactor in native form.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 39, Issue 3, 1 May 2008, Pages 450–456
نویسندگان
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