کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4432037 1619912 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Long-term genotoxic effect of monocrotophos in different tissues of freshwater fish Channa punctatus (Bloch) using alkaline single cell gel electrophoresis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Long-term genotoxic effect of monocrotophos in different tissues of freshwater fish Channa punctatus (Bloch) using alkaline single cell gel electrophoresis
چکیده انگلیسی

Monocrotophos, commonly known as azodrin, is one of the organophosphate pesticides extensively used in agricultural practices throughout the world. However, little information is available on its long-term genotoxic effects in different tissues of fish using genotoxicity biomarkers. The objective of the present study was to detect DNA damage, induced by monocrotophos in freshwater teleost fish Channa punctatus using the comet assay. The LC50-96 h value of technical-grade monocrotophos was estimated for the fish species in a semi-static system. On the basis of the LC50 value, the sublethal and nonlethal concentrations were determined. The DNA damage was measured in the gill, kidney and lymphocytes as the percentage of DNA in comet tails of fish exposed to different sublethal and nonlethal concentrations of monocrotophos. In general, significant effects (P < 0.01) from both concentration and time of exposure were observed in exposed fish. It was found that the tissues at all concentrations exhibited the highest DNA damage on day 4, after which there was a decline in percentage tail DNA. The comparison of DNA damage among tissues at different concentrations indicated that the gill cells were more sensitive to the pesticide than the kidney cells and lymphocytes. This study also explored the utility of the comet assay for in vivo laboratory studies using fish for screening the genotoxic potential of various agents.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Science of The Total Environment - Volume 405, Issues 1–3, 1 November 2008, Pages 345–350
نویسندگان
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