کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4516037 1322340 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study of kernel structure of high-amylose and wild-type rice by X-ray microtomography and SEM
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Study of kernel structure of high-amylose and wild-type rice by X-ray microtomography and SEM
چکیده انگلیسی

Rice kernels of a transgenic high-amylose rice line and its wild type were examined by a high-resolution X-ray microtomography (XMT) and an environmental scanning electron microscope. Two-dimensional (2-D) cross-sectional images and 3-D objects from XMT were reconstructed and analyzed to elucidate their structural features. The lack of two isoforms of starch branching enzyme, termed SBEI and SBEIIb in high-amylose rice (HAR), resulted in a distinctly different grain inner density than wild-type rice (WTR). HAR had smaller, elongated starch granules with air spaces inside the kernels resulting in an opaque grain, whereas WTR had a tight endosperm with little air space and polygonal starch granules. XMT allowed a full 3-D characterization of the rice kernel structure and revealed that air space distribution was not uniform in the HAR kernel.


► Rice with greater than 50% amylose content was developed.
► Ultrastructure of rice kernels was studied by X-ray microtomography.
► Images from X-ray microtomography were compared with scanning electron microscopy.
► High-amylose rice had more air spaces and gave an opaque appearance.
► Air space distribution was not uniform in the high-amylose rice kernel.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cereal Science - Volume 55, Issue 1, January 2012, Pages 1–5
نویسندگان
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