Abundance and distribution of fatty acids within the walls of an active deep-sea sulfide chimney

Abundance and distribution of total fatty acids (TFAs) were examined along the physicochemical gradient within an active hydrothermal chimney collected from the Main Endeavour segment of Juan de Fuca Ridge. Approximately 27 fatty acids are identified with a chain-length ranging from C12 to C22. From the exterior to the interior of the chimney walls, the total concentrations of TFAs (∑ TFAs) show a trend of evident decrease. The observed compositions of TFAs are rich in bacterial biomarkers especially monounsaturated fatty acids (MUFAs) and minor branched and cyclopropyl FAs. On the basis of the species-specific FAs and bacterial 16SrRNA gene analysis (Li et al., unpublished data), sulfur-based metabolism appears to be the essential metabolic process in the chimney. Furthermore, the sulfur oxidizing bacteria (SOB) are identified as a basic component of microbial communities at the exterior of the hydrothermal chimney, and its proportion shows an inward decrease while the sulfate reducing bacteria (SRB) have an inverse distribution.

Research Highlights
► Fatty acids were examined in an active vent chimney.
► The value of ∑ TFAs decreases from the exterior to the interior of the chimney wall.
► Microorganism with sulfur-based metabolism were dominant in the microbial communities.
► The proportion of SOB shows an inward decrease.
► The proportion of SRB has an inverse distribution compared to SOB.