Enhancement of curcumin water dispersibility and antioxidant activity using core–shell protein–polysaccharide nanoparticles

• Curcumin-loaded core–shell nanoparticles were fabricated as delivery systems.
• The protein core was formed by antisolvent precipitation of zein.
• The polysaccharide shell was formed by electrostatic deposition of pectin or alginate.
• Pectin–alginate ratio impacted the physical stability of the nanoparticles.
• Optimized curcumin-loaded nanoparticles had good antioxidant activity.

Curcumin has strong antioxidant activity, but poor water-solubility and chemical stability, which limits its utilization as a nutraceutical in many applications. Previously, we developed a core–shell (zein–pectin) nanoparticle delivery system with high curcumin loading efficiency, high particle yield, and good water dispersibility. However, this system was unstable to aggregation around neutral pH and moderate ionic strengths due to weakening of electrostatic repulsion between nanoparticles. In the current study, we used a combination of alginate (high charge density) and pectin (low charge density) to form the shell around zein nanoparticles. Replacement of 30% of pectin with alginate greatly improved aggregation stability at pH 5 to 7 and at high ionic strengths (2000 mM NaCl). Curcumin encapsulated within these core–shell nanoparticles exhibited higher antioxidant and radical scavenging activities than curcumin solubilized in ethanol solutions as determined by Fe (III) reducing power, 1, 1-Diphenyl-2-picrylhydrazyl free radical (DPPH·), and 2, 2′-azinobis-(3-ethylbenzothiazoline)-6-sulfonic acid radical cation (ABTS·+) scavenging analysis. These core–shell nanoparticles may be useful for incorporating chemically unstable hydrophobic nutraceuticals such as curcumin into functional foods, dietary supplements, and pharmaceuticals.

Curcumin-loaded core–shell nanoparticles were fabricated by antisolvent precipitation of zein, followed by electrostatic deposition of pectin and/or alginate. The pectin–alginate ratio impacted the physical stability and antioxidant activity of the curcumin-loaded nanoparticles.Figure optionsDownload as PowerPoint slide