Evaluation of transglutaminase and caseinate for a novel formulation of beef patties enriched in healthier lipid and dietary fiber

The food intake in developed countries is characterized by a deficient consumption of n-3 fatty acids and fiber. In this study, modified beef patties enriched with polyunsaturated n-3 fatty acids and fiber were developed. The texture profile was compared to traditional beef patties, showing lower values for all parameters except springiness. To improve its texture, pretreatments using transglutaminase (TG) and sodium caseinate (C) were assayed by response surface methodology. Mathematical models were obtained and used to determine the effect of TG, C, temperature and time of pretreatment on the texture of beef patties. The overall optimization indicated that a pretreatment with 0.1 U/g of TG at 40 °C for 17 min allowed to have a traditional texture for beef patties enriched in n-3 PUFAs and fiber. The models showed that the addition of caseinate was not needed. Minimal effects of pretreatment were observed on composition, color and cooking loss of the product.