کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4565003 1330956 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A kinetic assessment of the enzymatic hydrolysis of potato (Solanum tuberosum)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
A kinetic assessment of the enzymatic hydrolysis of potato (Solanum tuberosum)
چکیده انگلیسی

The biotechnology industry demands raw materials to prepare growth media for fermentative processes. These media contain a carbon source which can be obtained from potato, an abundant source of starch. This work deals with the modelling of the enzymatic hydrolysis of potato using α -amylase and glucoamylase. Several strategies were evaluated, including the use of those enzymes alone, in a mixture and under different conditions of temperature, time and substrate concentration. Using α -amylase alone or glucoamylase alone, both enzymes reached the same hydrolysis yield (0.42 g/g). The hydrolysis using a mixture of both enzymes allowed obtaining hydrolysates with reducing sugar concentrations up to 14.1 g/L at 70 °C. In this case, the yield of hydrolysis was increased up to 0.82 g/g. Considering the important effect and interactions of time and temperature, a statistical Box–Behnken design was conducted including substrate concentration, time and temperature as operational variables and reducing sugar concentration released as dependent variable. The conditions to obtain the maximum response were 6% DM of substrate concentration, 68 °C and 180 min, where 32.62 g/L of reducing sugar concentration was predicted. Verification experiments gave a mean value of 33.57 g/L, confirming the accuracy of the model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 42, Issue 4, May 2009, Pages 797–804
نویسندگان
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