کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4736 | 241 | 2008 | 6 صفحه PDF | دانلود رایگان |

The thermostability of immobilized lipase from Candida antarctica in subcritical acetone, which was in the liquid state under pressurized conditions, was examined in the temperature range from 60 to 90 °C. The inactivation process of the enzyme did not obey first-order kinetics and the enzyme activity leveled off after a prolonged incubation. The level depended on the temperature, and the remaining activity at 80 °C was 15.4% after 1.5-h incubation. A reactor system consisting of columns packed with xylitol and the immobilized lipase, which were connected in series, was constructed and operated at 80 °C to continuously produce the xylitoyl fatty acid esters. Xylitoyl laurate, myristate and palmitate were synthesized at the constant conversion of ca. 70%, and the productivities of 512, 519 and 551 g/day/L-reactor, respectively, were realized.
Journal: Biochemical Engineering Journal - Volume 38, Issue 1, 15 January 2008, Pages 16–21