کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4752039 | 1415988 | 2017 | 43 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Production of (R)-(â)-mandelic acid with nitrilase immobilized on D155 resin modified by l-lysine
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Production of (R)-(â)-mandelic acid with nitrilase immobilized on D155 resin modified by l-lysine Production of (R)-(â)-mandelic acid with nitrilase immobilized on D155 resin modified by l-lysine](/preview/png/4752039.png)
چکیده انگلیسی
A novel immobilized nitrilase (I-N-l-D155) immobilized with D155 resin modified by l-lysine (L-D155) was synthesized and used in the production of (R)-(â)-mandelic acid ((R)-(â)-MA). FTIR spectra indicated that nitrilase has been introduced into L-D155 resin and the Schiff base was formed. The condition of mandelonitrile hydrolysis was optimized, the hydrolysate characteristics was executed. The characterizations of the hydrolysate illustrated the hydrolysate was (R)-(â)-mandelic acid. The hydrolysis yield of (R)-(â)-mandelic acid was 53.42 ± 0.63% with the enantiomeric excess above 99% for hydrolysis of mandelonitrile in 45 °C, pH = 9.6, I-N-l-D155/mandelonitrile = 0.5 for 5 h Km value and thermostability of I-N-l-D155 were increased by 22.74 ± 2.08% and 5 °C, respectively, compared to free nitrilase. And the enzyme activity was retained 92.48 ± 0.32% of its initial activity after continuous 15th reuse. This novel immobilized nitrilase showed potential application for (R)-(â)-mandelic acid production and might be a valuable strategy to facilitate efficiency and cost savings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 127, 15 November 2017, Pages 32-42
Journal: Biochemical Engineering Journal - Volume 127, 15 November 2017, Pages 32-42
نویسندگان
Wen Jiang, Renyou Tao, Yang Yang, Yang Xu, Hongkuan Kang, Xiaohua Zhou, Zhiming Zhou,